SPM Biology 2026 Negeri Sembilan Trial Set 1 Kertas 1 – 40 Questions with Answers

1

Which of the following biological waste should be sterilised in an autoclave before disposal?

A Syringe B Gloves C Animal tissue D Scalpel

2

Diagram 1 shows a plant cell. What is the structure labelled **Q**?

A Vacuole B Tonoplast C Nucleus D Lysosome

3

Diagram 2 shows the condition of a red blood cell after being immersed in a solution. What is the phenomenon shown by the cell?

A Crenation B Haemolysis C Flaccid D Plasmolysis

4

A housewife cut a pineapple into small slices and sprinkles sugar on the fruit slices. After 20 minutes, she noticed that the fruit slices become soft. Which of the following statement is correct about the condition?

A Water diffuses out from the pineapple cell by osmosis B Water diffuses into the pineapple cell by osmosis C Sugar is transported into the pineapple cell by active transport D Sugar is transported into the pineapple cell by facilitated diffusion

5

Diagram 3 shows a graph of the effect of substrate concentration on the rate of enzyme reaction. Which of the following conclusion is the best for the graph? I Enzyme concentration is the limiting factor at level Z II At concentration Y, no more reaction takes place as all the active sites of enzymes are fully saturated III When substrate concentration increases from 0 to Y, more substrate molecules bind with the active site of enzymes IV Rate of reaction at level Z can be increased if more substrate is added

A I and II B I and III C II and IV D III and IV

6

One molecule **K** combines with three molecules **L** by condensation process to produce one molecule **M** and three molecules **N**. Which of the following is correct for **K**, **L**, **M** and **N**?

A K: Glycerol; L: Fatty acid; M: Water; N: Triglyceride B K: Fatty acid; L: Glycerol; M: Triglyceride; N: Water C K: Glycerol; L: Fatty acid; M: Triglyceride; N: Water D K: Fatty acid; L: Glycerol; M: Water; N: Triglyceride

7

Diagram 4 shows a type of food produced using fermentation process. Which word equation shows the fermentation process that occur in the food?

A Glucose + Oxygen → Ethanol + Carbon dioxide + Energy B Glucose + Oxygen → Lactic acid + Energy + Water C Glucose → Ethanol + Carbon dioxide + Energy D Glucose → Lactic acid + Energy

8

Diagram 5 shows a stage of a cell division in the reproductive organ of an animal. Which diagram represents the gamete cell after the cell division is completed?

A

B

C

D

9

The following word equation shows a process that occurs in organ **P** in the human digestive system. > Polypeptide + water —(Enzyme Q)→ R What is organ **P**, enzyme **Q** and **R**?

A P: Stomach; Q: Pepsin; R: Peptide B P: Mouth; Q: Salivary amilase; R: Maltose C P: Duodenum; Q: Trypsin; R: Peptide D P: Duodenum; Q: Erypsin; R: Amino acids

10

Diagram 6 shows the human respiratory system. What changes happen to **Q** during inhalation?

A Contracts, becomes flat and horizontal B Contract and curves upwards to form a dome C Relaxes, becomes flat and horizontal D Relaxes and curves upwards to form a dome

Negeri Sembilan Trial 2026 Biology Kertas 1 · Set 1

Negeri Sembilan Trial 2026 Biology Kertas 1 · Set 1

Negeri Sembilan Trial 2026 Biology Kertas 1 · Set 1

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